A Blood Sugar Test Method is a procedure that measures the amount of sugar, or glucose, in your blood. Your doctor may order this test to help diagnose diabetes.
Also Known as: Fasting Blood Sugar, FBS, Fasting Blood Glucose, Blood Sugar Fasting, BSF, BSL
Test Panel: Blood Sugar, Fasting Blood Sugar, FBS, Fasting Blood Glucose, FBG, Fasting Plasma Glucose, FPG, Blood Glucose, Oral Glucose Tolerance Test, OGTT, GTT, Urine Glucose
Principle:
Glucose is oxidised to gluconic acid and hydrogen peroxide in the presence of glucose oxidase. Hydrogen peroxide further reacts with phenol and 4 – aminoantipyrine by the catalytic action of peroxidase to form a red coloured quinoneimine dye complex. Intensity of the colour formed is directly proportional to the amount of glucose present in the sample.
Kit Content:
| L1 : Glucose Reagent | 2 x 150 ml, 3 x 150 ml, 1 x 1000 ml |
| S : Glucose Standard (100 mg/di) | 5.0 ml |
Reagent Storage I stability
Contents are stable at 2-8°C till the expiry mentioned on the labels. Upon storage the Glucose reagent may develop a slight pink colour. This does not affect the performance of the test.
Reagent Preparation:
Reagents are ready to use.
Sample Required:
Serum, plasma, CSF. Glucose is reported to be stable in the sample for 7 days when stored at 2-8°C.
System Parameters:
| Reaction | End Point |
| Wavelength / filter | 505 nm (Hg 546 nm)/ Green |
| Zero Setting | Reagent Blank |
| lncub. Temperature | 37°C / R. T. |
| lncub. Time | 10 min. / 30 min. |
| Light path | 1cm |
| Volume | 400 μL |
| Standard | 100 mg/dl |
| React. Slope | Increasing |
| Linearity | 500 mg/dl |
| Units | mg/dl |
Procedure:
Pipette into clean dry test tubes labelled as Blank (B), Standard (S), and Test (T):
| Additiion Sequence | B (Blank) | S (Standard) | T (Test) |
|---|---|---|---|
| Glucose Reagent (L1) | 1000 μL | 1000 μL | 1000 μL |
| Distilled water | 010 μL | ||
| Glucose Standard (S) | 10 μL | ||
| Sample | 10 μL |
Mix well and incubate at 37°C for 10 min. or at R.T. (25°C) for 30 min. Measure the absorbance of the Standard (Abs.S), and Test Sample (Abs.T) against the Blank, within 60 Min.
Test Calculation:
Sugar Normal Values :
| Blood Glucose Test | Normal Range |
|---|---|
| Fasting Blood Glucose (FBS) | 70-99 mg/dL (3.9-5.5 mmol/L) |
| Random Blood Glucose (RBS) | 90-140 mg/dL (7.8 mmol/L) |
| OGTT (5 Samples) | Fasting | 70-99 mg/dL (3.9-5.5 mmol/L) 30-Minutes| 80 – 130 mg/dL (3.9-7.2 mmol/L) 60-Minutes | 110 – 180 mg/dL (3.9-9.99 mmol/L) 90-Minutes | 100 – 150 mg/dL (3.9-8.2 mmol/L) 120-Minutes | 90 – 140 mg/dL (3.9-7.8 mmol/L) |
| HbA1c | Upto 5.7% |
Linearity:
This procedure is linear upto 500 mg/di. If values exceed this limit, dilute the serum with normal saline (NaCl 0.9%) and repeat the assay. Calculate the value using the proper dilution factor.
Possible References Used
