Sudan Black B Staining 50 FAQs and 30 MCQs
Sudan Black B Staining 50 FAQs
What is Sudan Black B (SBB)?
SBB is a fat-soluble dye used to stain lipids, neutral fats, and phospholipids in cells.
What is the primary use of Sudan Black B staining?
It is used to differentiate Acute Myeloid Leukemia (AML) from Acute Lymphoid Leukemia (ALL) and to stain lipids in histology and cytology.
How does SBB staining compare to Myeloperoxidase (MPO) staining?
SBB can stain older smears, stains both azurophilic and specific granules, and shows less fading over time compared to MPO.
What types of lipids does SBB stain?
SBB stains neutral fats, sterols, phospholipids, and triglycerides.
What cells are typically SBB-positive?
Myelocytic cells (azurophilic and secondary granules) and monocytic cells (lysosomal granules) are SBB-positive.
What cells are typically SBB-negative?
Lymphoid cells are generally SBB-negative, though less than 3% of blast cells in ALL may show positivity.
Can SBB be used for frozen histology sections?
Yes, SBB is used to stain lipids in frozen histology sections.
What is the stability of SBB dye solution?
The solution is stable for 1 year when stored at 60°C.
What is the role of propylene glycol in SBB staining?
Propylene glycol is used as a solvent for SBB dye and as a differentiating agent.
What is the purpose of Nuclear Fast Red in SBB staining?
Nuclear Fast Red is used as a counterstain to highlight nuclei in red.
What type of sample is required for SBB staining?
Fresh anticoagulated whole blood or bone marrow smears are used.
How should slides be fixed for SBB staining?
Slides should be fixed in 40% formaldehyde vapor, formalin, or formalin-ethanol for 10 minutes.
Can SBB stain older smears?
Yes, SBB can be used on smears more than 2 weeks old.
What is the fixative used in SBB staining?
40% formaldehyde solution vapor is commonly used.
How long should slides be washed after fixation?
Slides should be washed gently in water for 5-10 minutes.
What is the purpose of air-drying smears before staining?
Air-drying ensures proper fixation and adherence of the sample to the slide.
Can frozen sections be stained with SBB?
Yes, frozen sections can be stained using the SBB lipid staining method.
What is the role of tap water in the staining process?
Tap water is used to rinse slides after fixation and staining steps.
How should samples be collected for SBB staining?
Samples should be collected using appropriate instruments and processed with modern technology.
What precautions should be taken during sample preparation?
Follow manufacturer instructions and ensure samples are clearly marked to avoid errors.
Follow manufacturer instructions and ensure samples are clearly marked to avoid errors.
Follow manufacturer instructions and ensure samples are clearly marked to avoid errors.
What is the composition of the working SBB stain solution?
It consists of 40 ml phenol buffer and 60 ml SBB solution.
What is the purpose of 70% alcohol in SBB staining?
70% alcohol is used to differentiate the stain by removing excess dye.
How many times should 70% alcohol be applied?
It should be applied three times, each for 30 seconds.
What counterstains can be used with SBB?
May-Grunwald-Giemsa or Leishman stain can be used as counterstains.
How long should slides be counterstained?
Counterstaining time depends on the stain used but typically takes a few minutes.
What is the role of phenol buffer in SBB staining?
Phenol buffer helps maintain the pH and stability of the staining solution.
How should SBB solution be prepared?
Dissolve 0.3 g SBB in 100 ml absolute ethanol and mix with phenol buffer.
What temperature should SBB solution be heated to for frozen sections?
The solution should be heated to 100°C during preparation.
How long should frozen sections be stained with SBB?
Frozen sections should be stained for 6-10 minutes.
What does a positive SBB reaction look like?
A positive reaction shows black or granular pigment in the cells.
What does SBB positivity indicate in myelocytic cells?
It indicates the presence of lipids in azurophilic and secondary granules.
How does SBB staining intensity change with cell maturity?
Staining intensity increases as cells mature from myeloblasts to mature forms.
What color do nuclei appear after counterstaining?
Nuclei appear red when counterstained with Nuclear Fast Red.
What does blue-black staining indicate in SBB results?
Blue-black staining indicates the presence of lipids.
What does red staining indicate in SBB results?
Red staining indicates nuclei after counterstaining.
Are basophils SBB-positive?
Basophils are generally not SBB-positive but may show metachromatic staining.
How do monocytic cells react to SBB staining?
Monocytic cells show variable reactions, from negative to weakly positive.
What does SBB negativity in lymphoid cells indicate?
SBB negativity helps differentiate lymphoid cells from myeloid cells.
What is the significance of less than 3% SBB positivity in ALL?
It indicates rare blast cells that may show positivity, but lymphoid cells are generally negative.
How should SBB powder dye be stored?
It should be stored in a tightly closed original package at 15-25°C, away from direct sunlight.
What is the shelf life of SBB powder dye?
The expiry date is printed on the label, but the dye solution is stable for 1 year at 60°C.
What safety precautions should be taken during SBB staining?
Handle chemicals with care, follow safety guidelines, and dispose of waste as special waste.
How should used SBB solutions be disposed of?
They should be disposed of as special waste according to national guidelines.
What are the risks associated with SBB staining?
Chemicals used in the procedure can be hazardous, and tissue specimens may be infectious.
What personal protective equipment (PPE) is recommended for SBB staining?
Gloves, lab coats, and safety goggles should be worn.
What should be done if SBB solution comes into contact with skin?
Wash the affected area immediately with soap and water.
Can SBB staining be performed by untrained personnel?
No, only authorized and qualified personnel should perform the staining and diagnostics.
What is the role of BioGnost’s material safety data sheet?
It provides safety information and handling instructions for SBB and related products.
What environmental precautions should be taken during SBB staining?
Follow environmental protection guidelines and avoid releasing chemicals into the environment.
Sudan Black B Staining 30 MCQs
- What is Sudan Black B primarily used for?
a) Staining proteins
b) Differentiating AML from ALL ✔
c) Identifying viral infections
d) Detecting carbohydrates - Which of the following is NOT stained by Sudan Black B?
a) Neutral fats
b) Phospholipids
c) Proteins ✔
d) Sterols - What is the advantage of SBB over Myeloperoxidase (MPO) staining?
a) SBB stains proteins more effectively
b) SBB can stain older smears ✔
c) SBB is water-soluble
d) SBB is cheaper - Which cells are typically SBB-positive?
a) Lymphoid cells
b) Myelocytic cells ✔
c) Erythrocytes
d) Platelets - What is the primary solvent used in SBB staining?
a) Water
b) Ethanol
c) Propylene glycol ✔
d) Acetone
- What type of sample is required for SBB staining?
a) Urine
b) Fresh anticoagulated whole blood or bone marrow smear ✔
c) Saliva
d) Stool - How long should slides be fixed in formalin vapor for SBB staining?
a) 1 minute
b) 5 minutes
c) 10 minutes ✔
d) 30 minutes - What is the purpose of air-drying smears before staining?
a) To evaporate water
b) To ensure proper fixation and adherence ✔
c) To remove lipids
d) To enhance protein staining - Which of the following is NOT a fixative used in SBB staining?
a) 40% formaldehyde vapor
b) Formalin-ethanol
c) Acetic acid ✔
d) Neutral buffered formalin - What is the role of tap water in the staining process?
a) To dissolve the dye
b) To rinse slides after fixation and staining ✔
c) To enhance lipid staining
d) To dehydrate the sample
- How long should slides be stained in SBB solution?
a) 10 minutes
b) 30 minutes
c) 1 hour ✔
d) 2 hours - What is the composition of the working SBB stain solution?
a) 40 ml SBB solution + 60 ml buffer
b) 60 ml SBB solution + 40 ml buffer ✔
c) 50 ml SBB solution + 50 ml buffer
d) 70 ml SBB solution + 30 ml buffer - What is the purpose of 70% alcohol in SBB staining?
a) To fix the sample
b) To differentiate the stain by removing excess dye ✔
c) To enhance lipid staining
d) To dehydrate the sample - Which counterstain is commonly used with SBB?
a) Hematoxylin
b) Eosin
c) May-Grunwald-Giemsa ✔
d) Crystal violet - What temperature should SBB solution be heated to for frozen sections?
a) 37°C
b) 60°C
c) 100°C ✔
d) 120°C
- What does a positive SBB reaction look like?
a) Blue granules
b) Black or granular pigment✔
c) Red nuclei
d) Green cytoplasm - What does SBB positivity indicate in myelocytic cells?
a) Presence of carbohydrates
b) Presence of lipids in azurophilic and secondary granules ✔
c) Presence of proteins
d) Presence of nucleic acids - What color do nuclei appear after counterstaining with Nuclear Fast Red?
a) Blue
b) Black
c) Red ✔
d) Green - Which cells are generally SBB-negative?
a) Myelocytic cells
b) Monocytic cells
c) Lymphoid cells ✔
d) Basophils - What does blue-black staining indicate in SBB results?
a) Nuclei
b) Lipids✔
c) Proteins
d) Carbohydrates
- How should SBB powder dye be stored?
a) In a freezer
b) In a tightly closed original package at 15-25°C ✔
c) In direct sunlight
d) In water - What is the shelf life of SBB dye solution?
a) 1 month
b) 6 months
c) 1 year ✔
d) 2 years - How should used SBB solutions be disposed of?
a) Pour down the sink
b) Dispose of as special waste ✔
c) Reuse for other experiments
d) Burn in an open flame - What PPE is recommended for SBB staining?
a) Gloves and lab coat ✔
b) Sunglasses
c) Sandals
d) No PPE required - What should be done if SBB solution comes into contact with skin?
a) Ignore it
b) Wash with soap and water✔
c) Apply alcohol
d) Cover with a bandage
- What is the role of propylene glycol in SBB staining?
a) To fix the sample
b) To dissolve the dye and act as a differentiating agent ✔
c) To dehydrate the sample
d) To enhance protein staining - Which of the following is NOT a lipid stained by SBB?
a) Neutral fats
b) Phospholipids
c) Sterols
d) Proteins ✔ - What is the purpose of Nuclear Fast Red in SBB staining?
a) To stain lipids
b) To counterstain nuclei ✔
c) To fix the sample
d) To differentiate the stain - What is the stability of SBB dye solution?
a) 6 months at room temperature
b) 1 year at 60°C ✔
c) 2 years at 4°C
d) Indefinitely - What is the primary use of SBB in hematology?
a) To detect viral infections
b) To differentiate AML from ALL ✔
c) To identify bacterial infections
d) To measure hemoglobin levels
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