Romanowsky Staining 50 FAQs and 30 MCQs:
Romanowsky Staining 50 FAQs
What are Romanowsky stains?
Romanowsky stains are used in hematology and cytology to differentiate cells and identify blood parasites.
Who developed Romanowsky stains?
They were developed by Dmitri Leonidovich Romanowsky, a Russian physician.
What is the principle behind Romanowsky stains?
They use a combination of acidic (eosin) and basic (methylene blue/azure) dyes to stain cellular components.
What is the Romanowsky effect?
What is the Romanowsky effect?
What are the main types of Romanowsky stains?
Giemsa, Wright, Wright-Giemsa, May-Grünwald, and Leishman stains.
Giemsa, Wright, Wright-Giemsa, May-Grünwald, and Leishman stains.
They are used for cellular definition, identifying blood parasites, and diagnosing hematological and cytological disorders.
What are the components of Romanowsky stains?
They typically consist of eosin (acidic dye) and methylene blue or its derivatives (basic dyes).
They typically consist of eosin (acidic dye) and methylene blue or its derivatives (basic dyes).
It is methylene blue that has undergone oxidative demethylation, producing multiple dyes like azure A, B, and C.
What is metachromasia?
It is the phenomenon where stains produce different colors in different cellular components.
What are the applications of Romanowsky stains?
They are used in hematology, cytology, and diagnosing blood parasites like malaria.
What is Giemsa stain used for?
It is used to examine blood films for parasitic infections like malaria and differentiate blood cells.
What dyes are in Giemsa stain?
It contains eosin Y (acidic) and methylene blue (basic).
How does Giemsa stain work?
It stains nuclei blue-purple and cytoplasm red-pink.
What is the procedure for Giemsa staining?
Fix the slide in methanol, flood with Giemsa stain, wait 30 minutes, rinse, and air dry.
What diseases can Giemsa stain diagnose?
Malaria, Chlamydia trachomatis, Borrelia spp., Yersinia pestis, and Pneumocystis jiroveci.
Is Giemsa stain used in cytogenetics?
Yes, it is used to detect chromosomal defects.
What is the difference between Giemsa and Wright-Giemsa stains?
Wright-Giemsa stain combines Wright and Giemsa stains for brighter cytoplasmic granules.
How long does Giemsa staining take?
Typically 30 minutes.
What is the role of methanol in Giemsa staining?
It acts as a fixative.
Can Giemsa stain be used for urine samples?
Yes, to detect urinary tract infections and interstitial nephritis.
What is Wright stain?
A modification of Romanowsky stain using heated methylene blue and eosin Y.
Who developed Wright stain?
James Homer Wright.
What is Wright-Giemsa stain?
A combination of Wright and Giemsa stains for enhanced staining.
What is the difference between Wright and Giemsa stains?
Wright stain uses heated methylene blue, while Giemsa uses standard methylene blue.
What is the purpose of Wright-Giemsa stain?
To differentiate blood cells and diagnose parasitic infections.
What are buffered Wright and Wright-Giemsa stains?
They use buffered solutions for staining.
How does Wright-Giemsa stain improve staining?
It produces brighter reddish-purple cytoplasmic granules.
What samples can Wright-Giemsa stain be used on?
Peripheral blood smears, bone marrow aspirates, and urine samples.
What is the duration of Wright-Giemsa staining?
It is faster than May-Grünwald-Giemsa staining.
What is the difference between Wright-Giemsa and May-Grünwald-Giemsa stains?
May-Grünwald-Giemsa produces more intense colors but takes longer.
What is May-Grünwald-Giemsa stain?
A two-step stain combining May-Grünwald and Giemsa stains.
What is the purpose of May-Grünwald-Giemsa stain?
To produce the Romanowsky effect for cellular differentiation.
How does May-Grünwald-Giemsa stain work?
It uses May-Grünwald stain first, followed by Giemsa stain.
What is the advantage of May-Grünwald-Giemsa stain?
It produces intense colors for better cellular definition.
What is the disadvantage of May-Grünwald-Giemsa stain?
It takes longer to perform.
What samples can May-Grünwald-Giemsa stain be used on?
Blood and bone marrow samples.
How does May-Grünwald-Giemsa stain differ from Giemsa stain?
It is a two-step process and produces more intense colors.
What is the role of May-Grünwald stain in the process?
It is the first step in the staining procedure.
What is the duration of May-Grünwald-Giemsa staining?
Longer than Wright-Giemsa staining.
What is the Romanowsky effect in May-Grünwald-Giemsa staining?
It produces a wide range of hues for cellular differentiation.
What is Leishman stain?
A Romanowsky stain using polychrome methylene blue and eosin Y.
Who developed Leishman stain?
William Leishman.
What is the purpose of Leishman stain?
To differentiate white blood cells and identify malaria parasites.
What is the role of methanol in Leishman stain?
It acts as a fixative.
What is the procedure for Leishman staining?
Cover the slide with stain, wait 1 minute, add water, wait 10-12 minutes, rinse, and air dry.
What samples can Leishman stain be used on?
Blood films for malaria and trypanosomes.
How does Leishman stain differ from Giemsa stain?
It uses polychrome methylene blue and has a different procedure.
What is the duration of Leishman staining?
Approximately 12-15 minutes.
What is the role of distilled water in Leishman staining?
It dilutes the stain for proper staining.
What is the advantage of Leishman stain?
It provides clear differentiation of white blood cells and parasites.
Romanowsky Staining 30 MCQs
- What are Romanowsky stains primarily used for?
a) Staining plant tissues
b) Differentiating cells and identifying blood parasites✔
c) Staining bacterial colonies
d) Identifying fungal infections - Who developed Romanowsky stains?
a) Louis Pasteur
b) Dmitri Leonidovich Romanowsky✔
c) Robert Koch
d) James Homer Wright - What is the Romanowsky effect?
a) The ability to stain only acidic tissues
b) The production of a variety of hues for cellular differentiation✔
c) The breakdown of methylene blue into multiple dyes
d) The fixation of cells using methanol - Which of the following is NOT a Romanowsky stain?
a) Giemsa stain
b) Gram stain✔
c) Wright stain
d) Leishman stain - What is the primary purpose of eosin in Romanowsky stains?
a) To stain acidic tissues red✔
b) To stain basic tissues blue
c) To fix the cells
d) To act as a solvent
- What is Giemsa stain primarily used for?
a) Staining bacterial cells
b) Diagnosing malaria and differentiating blood cells✔
c) Identifying fungal infections
d) Staining plant tissues - Which dyes are used in Giemsa stain?
a) Eosin Y and methylene blue✔
b) Hematoxylin and eosin
c) Safranin and crystal violet
d) Methylene blue and iodine - How long does Giemsa staining typically take?
a) 5 minutes
b) 10 minutes
c) 30 minutes✔
d) 1 hour - What is the role of methanol in Giemsa staining?
a) To act as a solvent
b) To fix the cells✔
c) To enhance the color
d) To dilute the stain - Which of the following diseases can Giemsa stain diagnose?
a) Tuberculosis
b) Malaria✔
c) HIV
d) Hepatitis
- Who developed Wright stain?
a) Dmitri Romanowsky
b) William Leishman
c) James Homer Wright✔
d) Louis Pasteur - What is the main difference between Wright and Giemsa stains?
a) Wright stain uses heated methylene blue✔
b) Giemsa stain uses eosin only
c) Wright stain is used for fungal infections
d) Giemsa stain is faster - What is Wright-Giemsa stain used for?
a) Staining plant tissues
b) Differentiating blood cells and diagnosing parasitic infections✔
c) Identifying bacterial colonies
d) Staining fungal cells - What is the advantage of Wright-Giemsa stain over Giemsa stain?
a) It is cheaper
b) It produces brighter reddish-purple cytoplasmic granules✔
c) It is faster
d) It requires no fixation - Which of the following is NOT a component of Wright stain?
a) Eosin Y
b) Heated methylene blue
c) Methanol
d) Safranin✔
- What is May-Grünwald-Giemsa stain used for?
a) Staining bacterial cells
b) Producing the Romanowsky effect for cellular differentiation✔
c) Identifying fungal infections
d) Staining plant tissues - What is the first step in May-Grünwald-Giemsa staining?
a) Fixation with methanol
b) Staining with May-Grünwald stain✔
c) Staining with Giemsa stain
d) Rinsing with water - What is the disadvantage of May-Grünwald-Giemsa stain?
a) It is expensive
b) It takes longer to perform✔
c) It produces faint colors
d) It cannot differentiate cells - What is the Romanowsky effect in May-Grünwald-Giemsa staining?
a) The breakdown of methylene blue
b) The production of a wide range of hues for cellular differentiation✔
c) The fixation of cells
d) The dilution of the stain - Which of the following is NOT a component of May-Grünwald-Giemsa stain?
a) Eosin Y
b) Methylene blue
c) Safranin✔
d) May-Grünwald stain
- Who developed Leishman stain?
a) Dmitri Romanowsky
b) William Leishman✔
c) James Homer Wright
d) Louis Pasteur - What is Leishman stain primarily used for?
a) Staining bacterial cells
b) Differentiating white blood cells and identifying malaria parasites✔
c) Identifying fungal infections
d) Staining plant tissues - What is the role of methanol in Leishman staining?
a) To act as a solvent
b) To fix the cells✔
c) To enhance the color
d) To dilute the stain - How long does Leishman staining typically take?
a) 5 minutes
b) 10-12 minutes✔
c) 30 minutes
d) 1 hour - What is the advantage of Leishman stain?
a) It is cheaper
b) It provides clear differentiation of white blood cells and parasites✔
c) It is faster
d) It requires no fixation
- Which of the following is NOT an application of Romanowsky stains?
a) Diagnosing malaria
b) Identifying chromosomal defects
c) Staining bacterial colonies✔
d) Differentiating blood cells - Which stain is used to detect chromosomal defects?
a) Giemsa stain✔
b) Wright stain
c) Leishman stain
d) May-Grünwald-Giemsa stain - Which stain is used to diagnose urinary tract infections?
a) Giemsa stain
b) Wright-Giemsa stain✔
c) Leishman stain
d) May-Grünwald-Giemsa stain - Which stain is used to detect bone marrow defects?
a) Giemsa stain
b) Wright stain
c) Leishman stain
d) All of the above✔ - Which stain is used to identify trypanosomes?
a) Giemsa stain
b) Wright stain
c) Leishman stain✔
d) May-Grünwald-Giemsa stain
Possible References Used
