Burrow’s stain, also known as Burrow’s method, is a histological staining technique used to highlight specific cellular and tissue components. It is particularly used in the study of skin lesions and dermatopathology.
Uses of Burrow’s stain:
- Dermatopathology: Identifies and differentiates various skin lesions and conditions.
- Histological Analysis: Highlights specific cellular components and tissue structures in biopsy samples.
- Disease Diagnosis: Aids in diagnosing skin diseases and disorders based on staining patterns and tissue morphology.
- Research: Used in research settings to study skin-related diseases and their effects on tissue structure.
- Educational Purposes: Provides clarity in teaching histology and dermatopathology by demonstrating specific staining characteristics.
Composition of Burrow’s stain:
Here is the table showing the composition of Burrow’s stain to make 100 ml:
| Component | Quantity |
|---|---|
| Thionin | 0.02 g |
| Ethanol, absolute | 3 ml |
| Acetic acid, glacial | 3 ml |
| Distilled water | 94 ml |
Preparation of Burrow’s stain:
- Weigh the Thionin: Weigh 0.02 g (20 mg) of thionin. If a precise balance is unavailable, use a wet swab stick or the unburnt end of a match stick to transfer a small amount of powdered thionin.
- Add Ethanol and Acetic Acid: Measure 3 ml of absolute ethanol and 3 ml of glacial acetic acid. Add these to a 100 ml leakproof bottle. Mix until the thionin is completely dissolved.
Caution: Handle ethanol and glacial acetic acid with care. They are flammable and glacial acetic acid is also corrosive and has irritating vapors. Use in a well-ventilated area away from open flames. Do not use mouth-pipettes. - Add Distilled Water: Add 94 ml of distilled water to the bottle and mix well to complete the preparation.
- Label and Store: Label the bottle with the contents and date of preparation. Store the stain at 2–8 °C. Renew the stain every 3 months for optimal performance.
Precautions:
- Thionin: Handle with care; use gloves and lab coat to avoid skin contact and inhalation.
- Ethanol: Highly flammable; keep away from flames and heat. Use in a well-ventilated area.
- Glacial Acetic Acid: Corrosive and flammable; use PPE (gloves, goggles) and work in a well-ventilated area. Avoid inhaling vapors.
- General Safety: Follow all safety protocols, label containers clearly, and store chemicals properly.
- Preparation Area: Keep the area clean, use appropriate containers, and avoid contamination.
- Disposal: Dispose of chemical waste according to institutional or local guidelines; do not pour down the sink.
Uses of in Clinical Laboratories Burrow’s stain:
- Skin Biopsies: Analyzes and differentiates various skin lesions for diagnostic purposes.
- Dermatopathology: Helps in identifying specific skin diseases and conditions through detailed staining of tissue samples.
- Histological Studies: Enhances visualization of cellular and tissue components in histological preparations.
- Disease Diagnosis:Assists in diagnosing dermatological conditions based on staining patterns and morphological details.
- Research: Used in research to study skin diseases and the effects on tissue structure.
Possible References Used
